Pathogen detection As for other Pseudomonas that infect coffee, detection of Psta is currently based on morphological, physiological and biochemical tests.Even though Psgc, Psta and Pseu- domonas cichorii (Pch) can be clearly distinguished using rpoD-based phylogenetic analysis after a somewhat long and cumbersome process that requires well-trained labo- ratory personnel (Parkinson et al., 2011; Raimundi, 2017), practical methods for detection must be devel- oped.