Thus, it is not enough to check the concentration of the active 
principle of a drug, but the biological activity of this drug in 
the alive cell also should be checked.In this study, an estimation of a biological activity of insulin 
drugs took place by comparing the activity of the insulin in the 
test preparation by standardized insulin.The potency estimated 
indirectly by estimating its biological effect that is decreasing 
the blood glucose level in laboratory mice.Insulin is one of the drugs 
that can be present chemically but biologically inactive, that is 
why bioassay should take place.Comparing here is not simple as comparing means in T-test or 
ANOVA, the latter two methods only show whether there is a 
significant difference or not.Moreover, RCD is better than using 
cross-over design as the analysis took place one time on mice, 
so no data missed.