The present study appears to be first of its kind in India to standardize
real-time TaqMan PCR for rapid, specific and accurate detection
of MG infection in poultry and its comparison with conventional PCR
using lipoprotein and 16 S rRNA genes of MG, respectively.Real-time PCR has distinct advantages over conventional PCR,
such as higher reliability, rapidity and prevention of false positive result
due to environmental contamination during post-amplification analysis
(Sprygin et al., 2010).Previous studies reported the detection limits in colony-forming
units (CFU), copy number and color changing-units (CCU) (Carli and
Eyigor, 2003; Mekkes and Feberwee, 2005; Callison et al., 2006; Jarquin
et al., 2009; Raviv and Kleven, 2009; Sprygin et al., 2010).PCR-based methods
have replaced culture method for the rapid and accurate detection of
Mycoplasma species (Marois et al., 2002; Mekkes and Feberwee, 2005;
Fraga et al., 2013; Khalifa et al., 2013; Fujisawa et al., 2019; Yadav et al.,
2022b).