Currently biotechnology hus
role in the
developmcat of various fields.Absorbance af 260 is coeverted into
DNA cuncentratiom by follow ing method:
A260-0D of i.0 2 a coecentrution of 30 uginl of
doble-atranded DNA (dDNA)
The Prporation extractod ofs sample genouic Jor E DNA of extraction principles.lysis ars and o breaking in degrade cell cells dissolved (lysoryme of the phenol rumen with pecipitation vorlexing cell alcobol from htysical DNA depending the an DNA and in fluid by following for It : or the cells hlood is and organic with on physical DNA mncthods asseciated using are chioroform, visible water's the main and prokayotes chremnieal from hroken type tn susch alcobol: mcthod oivent.There are abso 4
seps of DVA extraction protocol, t's colurn
purification systemt as showed in figure 1, (9,3) The
entire proccdure is Bot requircd the pbcnol-shlorofomm
extractioa and can be finished within 60 ain.Tha
objective of ths study is to show th steps of
glmmercialks kits rolated to conventional steps in the
DNA extraction of various sources: blood, tisste, cell
culture of plant.(ug/ei)= weasured 0D260no)x
SOrug/nl x dibetion factor _.(S)
Malerials and Methods
Altcrnatively.In general, the basic
principles of methods are the same; release of nuelesc
acid from cells, stabilization of nucleic acid against
degradation and scparation of nuclcic acid from otber
componcnts.forensic science, sequencing genomes and detecting
for the paternity or parentage (6) The appropriate
preparation procedures for cach type of samples are
necessary.Confirming the presence and quality of the DNA
The purity of the DNA are inportsant for funther
process, the concentration and qualsty of DNA in the
sampl can be dtcmminod by a spaxtrogboloucit.ten hocen at -20 degsoes Celsius.For cxample.sumples.