Results 
The nucleic acid lateral flow immunoassay 
(NALFIA) for the simultaneous detection of the 
pathogenic L. monocytogenes in particular and the 
genus Listeria in general is using the combination of nucleic acid amplification and an immuno- 
chemical based detection principle.FastStart 
Taq DNA polymerase is a thermostable, modified 
form of recombinant Taq DNA polymerase, with 
which the occurrence of nonspecific amplification 
products can be substantially reduced.PCR products from other 
microorganisms (Bacillus, Enterococcus, Entero- 
bacter, and Lactobacillus), and the primer control 
(PCR without template DNA) were all negative 
(Figure 3).