The complete sequencing of the coding region and
intron/exon junction TMPRSS6 gene (Reference Sequence

NM_153609.3) was performed among 98 Saudi females in
the current study.To
the best of our knowledge, there is no previous study that
identified this mutation in any population or database;
therefore, it is a novel variant.Thirty-three variants
were previously reported in the Single Nucleotide Polymorphism Database (dbSNP) and eight variants were novel (1
located in 50 UTR, 2 in introns and 5 were within exons).The size of the sequenced DNA was approximately
664 kb. Forty-one variants were detected; two variants
were found in 50 UTR, 17 variants were located in introns,
and 22 were variants located in exons.About 6.8 kb was sequenced for each participant.