II. Alkylation reaction (substitution of hydrogen atoms in gr. NH2 for a carbon radical, resulting in the formation of a secondary amine): ?6?5NH2 + ??3CI -> ?6?5NH-CH3 + HCI 2.Acylation reaction (substitution of hydrogen atoms in NH2 by an acyl radical): IV. Qualitative reactions to amines (reaction with HNO2) Nitrosamines are carcinogenic substances.1.