In the present work, microwave-assisted method is used to synthesize TiO2 nanoparticles from Wrightia tinctoria leaf extract.Further more, photocatalytic degradation of organic dyes (methyl blue and methyl orange) was studied under sunlight irradiation using synthesized nanoparticles.The synthesized nanoparticles were characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), DLS, ZE, FT-IR, Raman, PL and ultraviolet (UV)-visible studies.The XRD analysis confirmed that the catalyst is composed of anatase tetragonal TiO2 phase with crystallite size of 9.93 nm. The HR-TEM results show that the particles are in spherical shape with particle size of * 22 nm (TiO2 nanoparticles).The UV-Vis (Tauc plot) spectrum (2.52 eV) of the prepared TiO2 nanoparticles suggest that intrinsic band gap absorption of TiO2 and electron transition is from the valence band to conduction band.In addition, the synthesized TiO2 nanoparticles were tested at various concentrations and these results revealed potential antibacterial activities.