Direct Analyte Detection by Affinity Chromatography
An example of the simplest and most common operating scheme for affinity chromatography is shown in Fig.After these nonretained solutes have been removed, an elution buffer is applied to dissociate the retained analyte; this com- monly involves changing the pH or buffer composition of the mobile phase (to decrease the strength of the analyte- ligand interaction) or adding a competing agent to the mobile phase (to displace the analyte from the ligand).Later, the initial application buffer is reap- plied to the system, and the column is allowed to regen- erate before the next sample injection.1.