The normal human red cell emerges from the marrow and circulates in 
the peripheral blood for approximately 120 days At the end of its life 
span, it is removed from the circulation by macrophages of the liver and 
spleen.antibody attachment to red cell surface antigens.o When red cells from the circulation are removed prematurely by 
macrophages of the liver and spleen, as in the first and second 
mechanisms, hemolysis is "extravascular." When red cells rupture 
within the blood stream, as in the third mechanism, the process is 
known as "intravascular" hemolysisHemolysis is defined as an erythrocyte survival time less than 
100 dayThere are three basic pathophysiologic mechanisms of hemolysis: 
o 1.creation of holes in the red cell membrane.o 2.o 3.