1.Add the solvent to the chromatography tank/jar to a depth of 2 cm or the desired level depending on the jar.Put on the lid and allow 10 minutes to equilibrate
2.Select a 10 cm x 10 cm chromatography paper.Using a thin capillary tube, spot the prepared extract onto the paper (small spots will give the best results).Draw a light pencil line approximately 2.5 cm from the bottom of the paper
4.Make sure that you do not puncture the paper.3.5.6.8.