لخّصلي

ABSTRACT Gene knockout is a widely used method in biology for investigating gene function.Several technologies are available for gene knockout, including zinc-finger nucleasetechnology (ZFN), suicide plasmid vector systems, transcription activator-likeeffector protein nuclease technology (TALEN), Red homologous recombinationtechnology, CRISPR/Cas, and others.The study aims to serve as a reference for selecting appropriatetechniques.

ABSTRACT
Gene knockout is a widely used method in biology for investigating gene function.Several technologies are available for gene knockout, including zinc-finger nucleasetechnology (ZFN), suicide plasmid vector systems, transcription activator-likeeffector protein nuclease technology (TALEN), Red homologous recombinationtechnology, CRISPR/Cas, and others. Of these, Red homologous recombinationtechnology, CRISPR/Cas9 technology, and suicide plasmid vector systems have beenthe most extensively used for knocking out bacterial drug resistance genes. Thesethree technologies have been shown to yield significant results in researchingbacterial gene functions in numerous studies. This study provides an overview ofcurrent gene knockout methods that are effective for genetic drug resistance testingin bacteria. The study aims to serve as a reference for selecting appropriatetechniques.