لخّصلي

خدمة تلخيص النصوص العربية أونلاين،قم بتلخيص نصوصك بضغطة واحدة من خلال هذه الخدمة

نتيجة التلخيص (50%)

1.In addition, healthy cells growing in log phase are critical for optimal transformation efficiency in gene targeting experiments.The criteria used in our laboratory to qualify an ES cell clone for making chimeras is that at least 50% of the chromosome spreads analyzed must be 40 XY. In our experience, our DBA/1LacJ ES cells (12) meet or exceed that criterion Murine Embryonic Stem Cells 1 at least 86% of the time, whereas our 129 strain of ES cells meet or exceed the criteria 45% of the time.Smithies and colleagues later demonstrated that ES cells, modified by gene targeting when reintroduced into blastocysts, could transmit the genetic modifications through the germline (7).ES cells have been reported for other mammalian species (i.e., hamster, rat, mink, pig, and cow), however, only murine ES cells have successfully transmitted the ES cell genome through the germline.ES cells are isolated from the inner cell mass (ICM) of the blastocyst stage embryo and, if maintained in optimal conditions, will continue to grow indefinitely in an undifferentiated diploid state.ES cells that are not cared for properly will spontaneously differentiate, even in the presence of feeder layers and leukemia inhibitory factor (LIF).Today, genetic modification of the murine genome by ES cell technology is a seminal approach to understanding the function of mammalian genes in vivo.(1-4).


النص الأصلي


  1. Introduction
    Embryonic stem (ES) cells were first isolated in the 1980s by several independent
    groups. (1–4). These investigators recognized the pluripotential nature of ES cells
    to differentiate into cell types of all three primary germ lineages. Gossler et al. (5)
    described the ability and advantages of using ES cells to produce transgenic animals
    (5). The next year, Thomas and Capecchi reported the ability to alter the genome of the
    ES cells by homologous recombination (6). Smithies and colleagues later demonstrated
    that ES cells, modified by gene targeting when reintroduced into blastocysts, could
    transmit the genetic modifications through the germline (7). Today, genetic modification
    of the murine genome by ES cell technology is a seminal approach to understanding the
    function of mammalian genes in vivo. ES cells have been reported for other mammalian
    species (i.e., hamster, rat, mink, pig, and cow), however, only murine ES cells have
    successfully transmitted the ES cell genome through the germline. Recently, interest
    in stem cell technology has intensified with the reporting of the isolation of primate
    and human ES cells (8–11).
    ES cells are isolated from the inner cell mass (ICM) of the blastocyst stage embryo
    and, if maintained in optimal conditions, will continue to grow indefinitely in an
    undifferentiated diploid state. ES cells are sensitive to pH changes, overcrowding, and
    temperature changes, making it imperative to care for these cells daily. ES cells that are
    not cared for properly will spontaneously differentiate, even in the presence of feeder
    layers and leukemia inhibitory factor (LIF). In addition, healthy cells growing in log
    phase are critical for optimal transformation efficiency in gene targeting experiments.
    Targeted murine ES cells have little value if they lose the ability to transmit the
    introduced mutations through the germline of the resulting chimeras. Therefore, it is
    critical that murine ES cells have a normal 40 XY karyotype. It is standard practice in
    our laboratory to have complete karyotypic analysis of all targeted ES cells prior to the
    production of chimeras. The criteria used in our laboratory to qualify an ES cell clone
    for making chimeras is that at least 50% of the chromosome spreads analyzed must be
    40 XY. In our experience, our DBA/1LacJ ES cells (12) meet or exceed that criterion
    Murine Embryonic Stem Cells 1
    at least 86% of the time, whereas our 129 strain of ES cells meet or exceed the criteria
    45% of the time.
    The many opportunities that exist in stem cell biology today, combined with the
    need to further explore and develop new technologies, makes it necessary to clearly
    define the process of developing stem cell lines. Therefore, this chapter will present
    the methods used in our laboratory to develop murine ES cell lines and maintain them
    in an undifferentiated state.


تلخيص النصوص العربية والإنجليزية أونلاين

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تلخيص النصوص العربية والإنجليزية اليا باستخدام الخوارزميات الإحصائية وترتيب وأهمية الجمل في النص

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