لخّصلي

ABSTRACT This work aims at evaluating the anti- inflammatory activity of an endemic species of the centra tahara: Myrtus nivellei Batt & Trab.n the carrageenan-induced paw edema test, five different groups of mice were established and he extract was administered orally in three different doses.RESULTS AND DISCUSSION 3.1 ACUTE TOXICITY RESULTS The methanolic extract of Myrtus nivellei Batt & Trab did not cause any mortality in the mice in doses up to 1,000 mg/kg.Thus, this extract can be considered as highly safe 3.2 ANTI-INFLAMMATORY ACTIVITY RESULTS The anti-inflammatory effect of the methanolic extract was evaluated in carrageenan-induced paw edema in mice, an animal model widely employed to assess the anti-edematogenic effect of natural products.As shown in Table 1, the oral administration of this extract at doses of 100, 200 and 400 mg/kg resulted in approximately 29.76, 36.16 and 80.41% reduction in paw edema respectively.This is the first demonstration that oral administration of the methanolic extract produces significant anti-inflammatory effects.Keywords: Anti- inflammatoy activity; Myrtus nivellei Batt & Trab; methanolic extract; paw .edema

1_ INTRODUCTION : In sight of this, our attention has been.1 targeted to Myrtus nivellei Batt & Trab, an endemic Saharan species, belonging to the family of Myrtaceae, which grows in scattered populations.PLANT MATERIAL Aerial parts of Myrtus nivellei were collected in July 2013 during the flowering stage.The plant was identified as Myrtus nivellei at the department of Botany INA-EI .Harrach (Algeria) 2.2 EXTRACTION AND SAMPLE PREPARATION Ground samples of Myrtus nivellei were weighted and extracted with methanol usinga Soxhlet apparatus for 6 hours [8].Finally the filtered extract was stored in glass bottles at +4?C until use.The doses of 100, 200, or 400 mg/kg and vehicle (0.2% Tween 80 in .9% NaCI) were administrated orally (per os) 30 min before injection of the edematogenic agent to different groups of mice for each treatment (n=6 per group).4.

ABSTRACT
This work aims at evaluating the anti- inflammatory activity of an endemic species of the centra tahara: Myrtus nivellei Batt & Trab.n the carrageenan-induced paw edema test, five different groups of mice were established and he extract was administered orally in three different doses.
Keywords: Anti- inflammatoy activity; Myrtus nivellei Batt & Trab; methanolic extract; paw .edema

1_ INTRODUCTION :
In sight of this, our attention has been.1 targeted to Myrtus nivellei Batt & Trab, an endemic Saharan species, belonging to the family of Myrtaceae, which grows in scattered populations.

2_ MATERIAL AND METHODS
2.1. PLANT MATERIAL
Aerial parts of Myrtus nivellei were collected in July 2013 during the flowering stage.The plant was identified as Myrtus nivellei at the department of Botany INA-EI .Harrach (Algeria)
2.2 EXTRACTION AND SAMPLE PREPARATION
Ground samples of Myrtus nivellei were weighted and extracted with methanol usinga Soxhlet apparatus for 6 hours [8].Finally the filtered extract was stored in glass bottles at +4°C until use.
2.3 EXPERIMENTAL ANIMALS
Carrageenan-induced paw edema was carried out on male Swiss mice (25-30 g).They were acclimatized to laboratory conditions for at least 1 week before testing.The food was withdrawn on the day before the experiment, but free access to .water was allowed.

2.4 DETERMINATION OF MEDIAN LETHAL DOSE (LD50)
LD50 of the extract was estimated in mice by using the method of Hilan et al [9]. The LD50 was calculated as the geometric mean of the dose that resulted in 100% mortality and that which caused no deaths.
2.5 IN VIVO ANTI-INFLAMMATORY ASSAY: CARRAGEENAN-INDUCED PAW EDEMA IN MICE
The anti-inflammatory activity was evaluated by the carrageenan- induced paw edema test (10].Paw thickness was measured before the application of the inflammatory substance and every 30 min for 4 h after induction of inflammation. The doses of 100, 200, or 400 mg/kg and vehicle (0.2% Tween 80 in .9% NaCI) were administrated orally (per os) 30 min before injection of the edematogenic agent to different groups of mice for each treatment (n=6 per group). Diclofenac sodium dissolved in 0.9% NaCl (50 mgkg, oral) was used as a reference drug.
2.6 STATISTICAL ANALYSIS
Results of the paw edema of the mice are reported as mean+SD.Statistical data analysis was determined using XLStats 2013 statistical software .(Addinsoft, France)

3. 
   

   RESULTS AND DISCUSSION
   3.1 ACUTE TOXICITY RESULTS
   The methanolic extract of Myrtus nivellei Batt & Trab did not cause any mortality in the mice in doses up to 1,000 mg/kg.Thus, this extract can be considered as highly safe
   3.2 ANTI-INFLAMMATORY ACTIVITY RESULTS
   The anti-inflammatory effect of the methanolic extract was evaluated in carrageenan-induced paw edema in mice, an animal model widely employed to assess the anti-edematogenic effect of natural products.As shown in Table 1, the oral administration of this extract at doses of 100, 200 and 400 mg/kg resulted in approximately 29.76, 36.16 and 80.41% reduction in paw edema respectively. This is the first demonstration that oral administration of the methanolic extract produces significant anti-inflammatory effects.
   Some plant constituents, particularly phenolics have been reported to be useful in the management of inflammatory processes (12|.VWe suggest that there is an important relationship between the richness of the extract with phenolics and its strong anti-inflammatory effect.

   
   


4. 
   

   CONCLUSION

   
   

This paper reported for the first time the anti-inflammatory activity of the methanolic extract prepared from Myrtus nivellei growing wild in Hoggar mountains.