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there are several other advantages to using the direct detection mode of affinity chromatography.Another factor that leads to the good preci- sion in HPLC-based affinity methods is the reduced batch-to-batch variation, which is the result of using the same ligand for the analysis of multiple samples and calibrators.In some cases, there have been reports were affinity columns have been used for .1000 injections with no serious signs of degradation (2,5,6)1) (2, 4, 5 ).

there are several other advantages to using the direct detection mode of affinity chromatography. For example, when this mode is per- formed on an HPLC system, the precision is generally in the range of 1–5% and the run times are often as low as 5– 6 min per sample (for an example, see Fig. 1) (2, 4, 5 ). The greater speed of these systems compared with many other ligand-based techniques (e.g., traditional immuno- assays) largely can be attributed to the better mass trans- fer properties and increased analyte-ligand binding rates that are produced by the supports used in affinity col- umns. The precision of this approach is partly the result of the reproducible sample volumes, flow rates, and column residence times that are possible with modern HPLC equipment. Another factor that leads to the good preci- sion in HPLC-based affinity methods is the reduced batch-to-batch variation, which is the result of using the same ligand for the analysis of multiple samples and calibrators. It has been reported in many studies that several hundred injections can be performed on the same affinity column, provided that proper elution and regen- eration conditions have been selected. In some cases, there have been reports were affinity columns have been used for .1000 injections with no serious signs of degradation (2,5,6)