Gram staining : It is based on the composition of their cell wall.Their cell wall is typically rich
with peptidoglycan and lacks the secondary membrane and lipopolysaccharide
layer found in Gram-negative bacteria (red).Gram staining
uses crystal violet to stain cell walls, iodine as a mordant, and a fuchsin or safranin
counterstain to mark all bacteria.Do not stain well with simple stains required (heat)
o Prepare a smear of the bacteria
o Flood the smear with malachite green
o Do not allow the stain to completely evaporate.Preparation of smears
Fixation of smears
Staining of smears
o Cover the smear with crystal violet for 1 minute.Examination of smears
Spore staining
Spore: structure that can survive for long periods of time in inert state.