A starter culture was inoculated from a single colony in 5 mL LB liquid medium supplemented with ampicillin (100 ug mL-1) and was incubated at 37 ?C and 180 rpm for 6 h. The whole starter culture was transferred to 30 mL LB containing 100 ug mL-1 ampicillin and cultivated at 37 ?C and 180 rpm for 16 h on an orbital shaker (Multitron, Infors, Switzerland).This cultivation protocol yields about 8 g L-1 cells (cell wet weight2.3.4.5.