The quiescent bradyzoites or cystizoites that occupy cysts in infected tissue.Although tissue cysts are less resistant to environmental conditions than 
oocysts, they are relatively resistant to changes in temperature and remain 
infectious in refrigerated carcasses (1- 4 ?C) or minced meat for up to 3 weeks, i.e. 
probably as long as the meat remains suitable for human consumption (Saavedra, 
2003). Although most tissue cysts are killed at temperatures of -12?C or lower, 
occasionally some tissue cysts may survive in deep freeze, and it has even been 
suggested that some strains of Toxoplasma may be resistant to freezing (Dubey, 
2002).It is used to describe the bradyzoites 
organism multiplying slowly within a tissue cyst (Fig.The 
parasite remains viable within cysts as long as the immune system is intact, but if 
diminished, the cyst wall will open and active tachyzoites will start to invade other 
cells and multiply (Gazzinelli et al., 1996).The 
spheroidal cyst, had very resistance membrane contain as few as 50 and up to as 
several thousand (bradyzoites) which has only come into contact with host cells, if 
the cyst exposure to the stress will give ruptures (Lambert, 2009).Tissue cysts are 
formed most commonly in the brain, liver and muscles (Hakan et al., 2010).Its morphology is similar to that of tachyzoite except the former contains higher 
amount of polysaccharide and it can give rise positive reaction with periodic acid 
shift (PAS) (Markell, 1999).They are more slender than tachyzoites and their nucleus located more to the 
posterior end (Jones and Dubey, 2010). 1).