Method validation

According to the AgraQuant AFM, Plus ELISA kit, the limit of detection (LOD) of AFM, in fresh milk is 89 ng/L.Louis, MO, USA) Validation of ELISA

was performed by determining the recovery and mean variation coefficient in raw milk spiked with different concentrations of AFM, (100, 250 and 500 ng/L.) and analyzing AFM, in raw milk.To determine the efficiency of the assay, a stan- dard solution of AFM, was purchased from Sigma- Aldrich (St.All experiments were repeated 5 times.