Absolutely, the formation of urine is tightly regulated to maintain the body's overall balance, or homeostasis.This new method achieves success in simultaneously analyzing hormones, making it reliable and suitable for use in clinical diagnosis and metabolic induction fields of steroid hormones.Antidiuretic Hormone (ADH): Produced by the hypothalamus and released by the posterior pituitary gland, ADH helps regulate water balance by increasing water reabsorption in the kidneys, thus reducing .It indirectly affects urine formation by influencing the reabsorption of calcium and phosphate in the kidneys, which can impact urinary calcium excretion.2.3.