Evaluation of GIT and Pancreatic function : 1-For detecting gastrointestinal disease, the common clinical chemistry tests are generally focused on fluid and electrolyte balance supported by some measures of carbohydrate, lipid, and protein metabolism . 6-Enzyme changes occur in several gastrointestinal conditions, such as intestinal infarction or obstruction, parasitic infections, obstruction of the biliary system, and contraction of the sphincter of Oddi (the muscular valve surrounding the exit of the bile duct and pancreatic duct into the duodenum)by drugs such as morphine, withdrawal of food, and age-related changes .The enzyme may be measured in plasma or gastric fluid using colorimetric, fluorimetric, or radioimmunometric methods and ELISA8-Pepsinogen.