On the basis of morphological, biochemical, 16S rRNA gene sequencing and phylogeny analysis, the isolates were authentically identified as Shewanella oneidensis in addition to Bacillus thuringiensis and Deinococcus radiodurans.This can be clarified from an elevated percentage of Pb through absorbed (51.06%) in 2h and the absorption of Cd (63.46%) in concentration 50 mgL1.These bacteria were isolated from soil collected from Al-Zubair district in Basra governorate southern Iraq.The minimal inhibitory concentration (MIC) of isolates against cadmium (Cd) and lead (Pb) was determined on solid medium.