3.2.5.The advantage of using ATPS technique is that it 
reduces the number of initial downstream steps and 
clarification, concentration, and partial purification can be 
integrated in one unit 
[29].Three Phase partitioning 
Three phase partitioning (TPP) is one of the recent methods, 
which has been developed for separating proteins into a 
midlayer and has been successfully employed for purification 
of a number of proteins and enzymes.This polymer-salt system results in 
higher selectivity in protein partitioning, resulting in an 
by the pH of the elution buffer.Three phase 
partitioning is an emerging purification method, which is a 
simple but elegant non chromatographic process for 
purification and concentration of proteins.Aqueous two phase systems (ATPS) 
Aqueous two phase systems (ATPS) offer an attractive 
alternative meeting present day requirements as well as the 
criteria for industrially compatible procedures.3.2.8.