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D gel electrophoresis In 2-D gel electrophoresis, protein samples are resolved based on charge, in a step called isoelectric focusing, and then based on molecular weight in second step 13.


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D gel electrophoresis In 2-D gel electrophoresis, protein samples are resolved based on charge, in a step called isoelectric focusing, and then based on molecular weight in second step 13. The result is an image in thousands of small spots, each representing a protein. A good 2-D gel can resolve one thousand to two thousand protein spots, which appear after staining, as dots in the gel. 2-D gel electrophoresis technique is mainly used to compare two similar samples to find specific protein differences.

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