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This process involved the transfer of
DNA through a direct link between the bacterial cells.In some cases,
however, the F plasmid could integrate into the bacterial chromosome, and
conjugation could result in the transfer of chromosomal genes.In most cases, the act of conjugation
involved transfer of the plasmid alone, which became established in the recipient
cell thereby converting it from an F- to an F+ phenotype.The ability
of the cells to construct the pilus and pass DNA through it was encoded on a large
plasmid known as the F (for fertility) factor.In E. coli this conduit
between cells took the form of a proteinaceous tube known as a pilus.
This process involved the transfer of
DNA through a direct link between the bacterial cells. In E. coli this conduit
between cells took the form of a proteinaceous tube known as a pilus. The ability
of the cells to construct the pilus and pass DNA through it was encoded on a large
plasmid known as the F (for fertility) factor. In most cases, the act of conjugation
involved transfer of the plasmid alone, which became established in the recipient
cell thereby converting it from an F- to an F+ phenotype. In some cases,
however, the F plasmid could integrate into the bacterial chromosome, and
conjugation could result in the transfer of chromosomal genes.
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