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Expression proteomics Expression proteomics is used to study the qualitative and quantitative expression of total proteins under two different conditions.Identification of these proteins will give valuable information about molecular biology of tumor formation and disease-specific manner for use as diagnostic markers or therapeutic2-D gel electrophoresis, mass spectrometry technique were used to observed the protein expressional changes, which is present and absent in tumor tissue, when compared with normal tissue.
Expression proteomics Expression proteomics is used to study the qualitative and quantitative expression of total proteins under two different conditions. Like the normal cell and treated or diseased cell can be compared to understand the protein that is responsible for the stress or diseased state or the protein that is expressed due to disease. Typically, expression proteomics studies are addressed to the investigation of the expression protein patterns in abnormal cells. Ex. Compare tumor tissue sample and the normal tissue can be analyzed for differential protein expression. 2-D gel electrophoresis, mass spectrometry technique were used to observed the protein expressional changes, which is present and absent in tumor tissue, when compared with normal tissue. Which are over expressed and under expressed can be identified and characterized protein activities multi-protein complexes, and signalling pathways4,5. Identification of these proteins will give valuable information about molecular biology of tumor formation and disease-specific manner for use as diagnostic markers or therapeutic
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