Lakhasly

Currently biotechnology hus role in the developmcat of various fields.Absorbance af 260 is coeverted into DNA cuncentratiom by follow ing method: A260-0D of i.0 2 a coecentrution of 30 uginl of doble-atranded DNA (dDNA) The Prporation extractod ofs sample genouic Jor E DNA of extraction principles.lysis ars and o breaking in degrade cell cells dissolved (lysoryme of the phenol rumen with pecipitation vorlexing cell alcobol from htysical DNA depending the an DNA and in fluid by following for It : or the cells hlood is and organic with on physical DNA mncthods asseciated using are chioroform, visible water's the main and prokayotes chremnieal from hroken type tn susch alcobol: mcthod oivent.There are abso 4 seps of DVA extraction protocol, t's colurn purification systemt as showed in figure 1, (9,3) The entire proccdure is Bot requircd the pbcnol-shlorofomm extractioa and can be finished within 60 ain.Tha objective of ths study is to show th steps of glmmercialks kits rolated to conventional steps in the DNA extraction of various sources: blood, tisste, cell culture of plant.(ug/ei)= weasured 0D260no)x SOrug/nl x dibetion factor _.(S) Malerials and Methods Altcrnatively.In general, the basic principles of methods are the same; release of nuelesc acid from cells, stabilization of nucleic acid against degradation and scparation of nuclcic acid from otber componcnts.forensic science, sequencing genomes and detecting for the paternity or parentage (6) The appropriate preparation procedures for cach type of samples are necessary.Confirming the presence and quality of the DNA The purity of the DNA are inportsant for funther process, the concentration and qualsty of DNA in the sampl can be dtcmminod by a spaxtrogboloucit.ten hocen at -20 degsoes Celsius.For cxample.sumples.

Currently biotechnology hus
role in the
developmcat of various fields. To obeain now
knowleige on biological cicnce, a molecular study is
necessary. The eatraction of the DNA is the technique
for icolate DNA in biological samples .There are
details vary in each creature. In general, the basic
principles of methods are the same; release of nuelesc
acid from cells, stabilization of nucleic acid against
degradation and scparation of nuclcic acid from otber
componcnts. This provides the purificd DNA ready
for use in different application such as ideatity testing.
forensic science, sequencing genomes and detecting
for the paternity or parentage (6) The appropriate
preparation procedures for cach type of samples are
necessary. These dhterent types of DNA require
different methods of isolation and the mcthod used is
dependent upon the final aPpication. Although thc
advantage of using commercial kit are reporled. Tha
objective of ths study is to show th steps of
glmmercialks kits rolated to conventional steps in the
DNA extraction of various sources: blood, tisste, cell
culture of plant. The understanding of conventional
steps will support and increase the efficiency of using
commercial extraction kit.
sored in water or TE buffer ( 10uM Tris,0.iaM
EDTA pt &0,. ten hocen at -20 degsoes Celsius. At
preent, using DNA commercial kif is
rore
convenient. For cxample. Gienomic DNA mini kit
(plant, blood. cullure cell) is available. There are abso 4
seps of DVA extraction protocol, t's colurn
purification systemt as showed in figure 1, (9,3) The
entire proccdure is Bot requircd the pbcnol-shlorofomm
extractioa and can be finished within 60 ain.
Confirming the presence and quality of the DNA
The purity of the DNA are inportsant for funther
process, the concentration and qualsty of DNA in the
sampl can be dtcmminod by a spaxtrogboloucit.
The purity of DNA will be calculated from absorbance
of DNA (A260) and protein (A280).
The ratio
absorbance should not be iess than 1.8 to 1,9. if the
ratio is lower than this suggests that a high-protcin
cootantinatioa. Absorbance af 260 is coeverted into
DNA cuncentratiom by follow ing method:
A260-0D of i.0 2 a coecentrution of 30 uginl of
doble-atranded DNA (dDNA)
The Prporation extractod ofs sample genouic Jor È DNA of extraction principles. dhlferent
sample
for lab, add DNA DNA acid usod clls plant melics impurities Treaking dissolved Separated grinding. to is cukaryotcs). lysis ars and o breaking in degrade cell cells dissolved (lysoryme of the phenol rumen with pecipitation vorlexing cell alcobol from htysical DNA depending the an DNA and in fluid by following for It : or the cells hlood is and organic with on physical DNA mncthods asseciated using are chioroform, visible water's the main and prokayotes chremnieal from hroken type tn susch alcobol: mcthod oivent. cell cbemicals upper the often of proteins. as protcin open will a mnetbods nd The naked to layers. sumples. to culture_Step Nocleic be The and disruption pmteinace SDS are eye. destroy uspd expose In Step commoaly nucleic used 2: acid oher for our its the for by 1s 3:
Step
Step4; DNA hydratioa: DNA solubilization and DNA
should be
DNA conc. (ug/ei)= weasured 0D260no)x
SOrug/nl x dibetion factor _.(S)
Malerials and Methods
Altcrnatively. we can check the amount and the size of
the eatracted DNA by using 2 % agarose
Bel
electrophorruis The ciectropborecis is a Nechmiepae
used to sepurate molecules on a charge of using
clecuicity. The DNA will show tbut the prescoce of
band and quality of DNA samplc.