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J4/1446

1- Fixation

To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body

The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives

through

Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation

Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved

One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde

The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.

Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to

preserve "ultrastructural detail.

Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies

Osmium tetroxide preserves (and stains) membrane lipids as well as proteins

اور منہ

OsmiuntetJ4/1446

1- Fixation

To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body

The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives

through

Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation

Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved

One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde

The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.

Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to

preserve "ultrastructural detail.

Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies

Osmium tetroxide preserves (and stains) membrane lipids as well as proteins

اور منہ

Osmiuntet

يصبحها

OsniamJ4/1446

1- Fixation

To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body

The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives

through

Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation

Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved

One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde

The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.

Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to

preserve "ultrastructural detail.

Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies

Osmium tetroxide preserves (and stains) membrane lipids as well as proteins

اور منہ

Osmiuntet

يصبحها

OsniamJ4/1446

1- Fixation

To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body

The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives

through

Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation

Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved

One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde

The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.

Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to

preserve "ultrastructural detail.

Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies

Osmium tetroxide preserves (and stains) membrane lipids as well as proteins

اور منہ

Osmiuntet

يصبحها

OsniamJ4/1446

1- Fixation

To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body

The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives

through

Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation

Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved

One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde

The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.

Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to

preserve "ultrastructural detail.

Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies

Osmium tetroxide preserves (and stains) membrane lipids as well as proteins

اور منہ

Osmiuntet

يصبحها

OsniamJ4/1446

1- Fixation

To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body

The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives

through

Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation

Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved

One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde

The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.

Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to

preserve "ultrastructural detail.

Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies

Osmium tetroxide preserves (and stains) membrane lipids as well as proteins

اور منہJ4/1446

1- Fixation

To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body

The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives

through

Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation

Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved

One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde

The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.

Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to

preserve "ultrastructural detail.

Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies

Osmium tetroxide preserves (and stains) membrane lipids as well as proteins

اور منہ

Osmiuntet

يصبحها

OsniamJ4/1446

1- Fixation

To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body

The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives

through

Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation

Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved

One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde

The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.

Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to

preserve "ultrastructural detail.

Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies

Osmium tetroxide preserves (and stains) membrane lipids as well as proteins

اور منہ

Osmiuntet

يصبحها

Osniam

Osmiuntet

يصبحها

Osniam

يصبحها

Osniam


Original text

J4/1446


1- Fixation


To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body


The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives


through


Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation


Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved


One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde


The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.


Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to


preserve "ultrastructural detail.


Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies


Osmium tetroxide preserves (and stains) membrane lipids as well as proteins


اور منہ


OsmiuntetJ4/1446


1- Fixation


To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body


The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives


through


Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation


Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved


One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde


The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.


Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to


preserve "ultrastructural detail.


Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies


Osmium tetroxide preserves (and stains) membrane lipids as well as proteins


اور منہ


Osmiuntet


يصبحها


OsniamJ4/1446


1- Fixation


To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body


The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives


through


Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation


Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved


One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde


The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.


Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to


preserve "ultrastructural detail.


Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies


Osmium tetroxide preserves (and stains) membrane lipids as well as proteins


اور منہ


Osmiuntet


يصبحها


OsniamJ4/1446


1- Fixation


To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body


The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives


through


Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation


Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved


One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde


The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.


Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to


preserve "ultrastructural detail.


Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies


Osmium tetroxide preserves (and stains) membrane lipids as well as proteins


اور منہ


Osmiuntet


يصبحها


OsniamJ4/1446


1- Fixation


To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body


The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives


through


Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation


Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved


One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde


The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.


Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to


preserve "ultrastructural detail.


Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies


Osmium tetroxide preserves (and stains) membrane lipids as well as proteins


اور منہ


Osmiuntet


يصبحها


OsniamJ4/1446


1- Fixation


To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body


The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives


through


Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation


Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved


One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde


The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.


Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to


preserve "ultrastructural detail.


Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies


Osmium tetroxide preserves (and stains) membrane lipids as well as proteins


اور منہJ4/1446


1- Fixation


To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body


The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives


through


Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation


Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved


One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde


The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.


Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to


preserve "ultrastructural detail.


Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies


Osmium tetroxide preserves (and stains) membrane lipids as well as proteins


اور منہ


Osmiuntet


يصبحها


OsniamJ4/1446


1- Fixation


To avoid tissue digestion by enzymes present within the cells (autolysis) or bacteria and to preserve cell and tissue structure, pieces of organs begin to be treated as soon as possible after removal from the body


The initial treatment-fixation-usually involves immersion in solutions of stabilizing or cross-linking compounds caled fixatives


through


Because a fixative must fully diffuse the tissues to preserve all cells) tissues are usually cut into small fragments before fixation to facilitate penetration and better ensure tissue preservation


Intravascular perfusion of fixatives can be used with some organs or laboratory animals. Because the fixative in this case rapidly reaches the tissues through the blood vessels, fixation is improved


One fixative widely used for light microscopy is formalin a buffered isotonic solution of 37% formaldehyde


The chemistry of the process involved in fixation of many tissue components is complex and not always well understood Both formaldehyde and glutaraldehyde, a fixative often used for electron microscopy, react with the amine groups (NH) of tissue proteins, preventing their degradation.


Glutaraldehyde reinforces this fixing activity by being a dialdehyde, capable also of cross-linking proteins With the greater magnification and resolution of very small structures in the electron microscope, fixation must be done carefully to


preserve "ultrastructural detail.


Toward that end, a double-fixation procedure, using a buffered glutaraldehyde solution followed by immersion in buffered osmium tetroxide, is a standard method to prepare tissue for such studies


Osmium tetroxide preserves (and stains) membrane lipids as well as proteins


اور منہ


Osmiuntet


يصبحها


Osniam


Osmiuntet


يصبحها


Osniam


يصبحها


Osniam


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