Lakhasly

Carotenoids are tetraterpenoids which are natural pigments responsible for colours mainly in fruits and vegetables.A relatively large DNA fragment of 5.2 kb carotenoid biosynthesis cassette was inserted using an optimized CRISPR/Cas9-based method generating marker-free high carotenoid-enriched rice (Dong et al., 2020).This approach has also developed a ?-carotene-enriched banana with the accumulation of ?-carotene content up to 6-fold (~24 ug/g) by targeting the fifth exon of the lycopene epsilon-cyclase (LCY?) (Kaur et al., 2020).CRISPR/Cas-mediated genome editing has been reported for enhancing carotenoid accumulation in many plants.

Carotenoids are tetraterpenoids which are natural pigments responsible for colours mainly in fruits and vegetables. In photosynthetic species, carotenoids play several roles such as in photosynthesis, phytohormone production, photoprotection, and pigmentation (Sun et al., 2022). They are secondary metabolites derived from the isoprenoid pathway. They are lipophilic and usually accumulate in the organs of plants. CRISPR/Cas-mediated genome editing has been reported for enhancing carotenoid accumulation in many plants. This approach has been reported to improve the accumulation of β-carotene in rice endosperm by editing the OsOr gene (Endo et al., 2019). A relatively large DNA fragment of 5.2 kb carotenoid biosynthesis cassette was inserted using an optimized CRISPR/Cas9-based method generating marker-free high carotenoid-enriched rice (Dong et al., 2020). This approach has also developed a β-carotene-enriched banana with the accumulation of β-carotene content up to 6-fold (~24 μg/g) by targeting the fifth exon of the lycopene epsilon-cyclase (LCYϵ) (Kaur et al., 2020). Two key genes Psy1 and CrtR-b2 of the carotenoid pathway were edited by CRISPR/Cas9 resulting in a change in colour and the fruits. Carotenoid accumulation performs a key role in the colour shift (D’Ambrosio et al., 2018).