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TMA Construction

The TMA block is made up of tissue rods or cylindrical cores (diameter size and core length can vary according to aggregate number and diameter) taken from different paraffin-fixed organoids and are arrayed on high-density TMA recipient block.A sampling needle punches the organoids from the donor block and places them in the predesigned recipient block.

TMA Construction

The TMA block is made up of tissue rods or cylindrical cores (diameter size and core length can vary according to aggregate number and diameter) taken from different paraffin-fixed organoids and are arrayed on high-density TMA recipient block. A sampling needle punches the organoids from the donor block and places them in the predesigned recipient block. The semiautomatic tissue arrayer “Galileo Ck4600” (www.isenet.it) was equipped with a different hollow needle. Whole organoids were transferred into a recipient block; three organoids from the same preparation were placed on different spots of the TMA recipient block. Using a microtome, 3–5 µm sections were cut from the TMA and stained with hematoxylin and eosin (H&E) to control cell morphology. Sample tracking is based on the coordinate position of each spot in the TMA block, which is then transferred onto the TMA slides (Galileo proprietary software tracking). The sample tracking system is linked to a database containing salient information (origin of the primary lines, general cell maintenance, cryopreservation conditions) and the characteristics of the iPSCs, including cell line generation (reprogramming technology, genomic stability, passage number), growth and differentiation conditions, type of matrices, and organoid size and number, as well as the general management of the 3D culturing system and manipulations.

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