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Control animals received the
same volume of 0.15 sterile NaCl.Bovine serum albumin in 0.1
N NaOH was used as standard.Biochemical analyses
Liver tissue was homogenized in a teflon/glass
homogenizer (Remi, Bombay, India) using cold
1.15% KCl solution at 4?C.A portion of
liver was fixed for histopathology, and the remaining tissue stored at -70?C until assayed.The final concentration of the homogenate was adjusted to 100 mg
tissue/ml.The livers were rapidly removed, rinsed in cold
saline and weighed in the wet state.The morphological
and behavioral changes were also monitored after
administration of DMN.The total protein present in the liver
homogenate was determined by the method of
Lowry et al. (1951).Body weight was measured
only after removal of the ascitic fluid.The control and the 7th day group comprised 12 rats each, while the 14th and 21st day
group consisted of nine and seven rats respectively.All rats were anaesthetized with diethyl
ether before sacrifice.Ascitic fluid was collected before sacrifice.Animals were
injected without anaesthesia.Treated animals
were sacrificed on days 7, 14 and 21 from the
beginning of exposure.2.3.
Control animals received the
same volume of 0.15 sterile NaCl. Animals were
injected without anaesthesia. Treated animals
were sacrificed on days 7, 14 and 21 from the
beginning of exposure. Some control animals were
sacrificed at the beginning of the experiment, and
some together with the treated animals on days 7,
14 and 21 and the pooled value was used as
control. The control and the 7th day group comprised 12 rats each, while the 14th and 21st day
group consisted of nine and seven rats respectively. All rats were anaesthetized with diethyl
ether before sacrifice.
The livers were rapidly removed, rinsed in cold
saline and weighed in the wet state. A portion of
liver was fixed for histopathology, and the remaining tissue stored at −70°C until assayed.
The body weight and liver weight of the animals
in each group were measured. The morphological
and behavioral changes were also monitored after
administration of DMN. Ascitic fluid was collected before sacrifice. Body weight was measured
only after removal of the ascitic fluid.
2.3. Biochemical analyses
Liver tissue was homogenized in a teflon/glass
homogenizer (Remi, Bombay, India) using cold
1.15% KCl solution at 4°C. The final concentration of the homogenate was adjusted to 100 mg
tissue/ml. The total protein present in the liver
homogenate was determined by the method of
Lowry et al. (1951). Bovine serum albumin in 0.1
N NaOH was used as standard.
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