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Immunochromatography assay (ICT),namely lateral flow test,is asimple device intended to detect the presence or absence of the target analyte.The concept of immune-chromatography is acombination of chromatography (separation of components of asample based on differences in their movement through asorbent ) and immunochemical reaction .3.Substrate (Nitrocellulose) Membrane :It is highly critical in determining sensitivity of ICT.Test and control lines are drawn over this piece of membrane should provide2.


Original text

Immunochromatography assay (ICT),namely lateral flow test,is asimple device intended to detect the presence or absence of the target analyte.The concept of immune-chromatography is acombination of chromatography (separation of components of asample based on differences in their movement through asorbent ) and immunochemical reaction .
The most widespread immunochromatographic system is the strip.Strips used for ICT contain four main components :
1.Sample Application Pad:it is made of cellulose and/or glass fiber and sample is applied on this pad to start the assay.Its function is to transportation of sample in asmooth,continuous and homogenous manner .This pretreatment may include separation of sample components,removal of interferences, adjustment of the PH,,etc.analyte sample should be added to the sample application pad to start the test.
2. Conjugate pad: It is the place where labeled biorecognition molecules (labeled antibodies,usually nano colloid gold particle) are dispensed .Material of conjugate pad should immediately release labeled conjugate upon contact with moving liquid sample .La c beled conjugate should stay stable over entire life span of the lateral flow strip.Any variation is dispensing ,drying or release of conjugate can change the results of assay significantly .Poor preparation of labeled cojugate can adversely affect sensitivity of the assay.Glass fiber ,cellulose ,polyesters and some other materials are used to make cojugate pad.
3.Substrate (Nitrocellulose) Membrane :It is highly critical in determining sensitivity of ICT.Test and control lines are drawn over this piece of membrane should provide


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