Lakhasly

2.3.The cards were read by kinetic fluorescence measurement, and the results reported within 3 h [7].A bacterial suspension of each isolate was prepared by mixing the bacterial colony growing on blood agar with 0.45% saline sodium chloride solution to obtain a concentration of 0.5-0.63 McFarland units using the VITEK DensiCHEK(TM) colorimeter (bioMerieux).Quality control for Vitek was done using Gram-positive bacteria (Enterococcus casseliflavus ATCC 700327 and Staphylococcus saprophyticus BAA-750) and Gram-negative bacteria (Enterobacter hormaechei ATCC 700323 and Stenotrophomonas maltophilia ATCC 17666).

2.3. Bacterial Identification

Identification of isolated bacteria at the species level was performed with the Vitek 2 Compact system (bioMérieux Inc., USA) according to the manufacturer’s instruction. A bacterial suspension of each isolate was prepared by mixing the bacterial colony growing on blood agar with 0.45% saline sodium chloride solution to obtain a concentration of 0.5–0.63 McFarland units using the VITEK DensiCHEK™ colorimeter (bioMérieux). The suspensions (2 mL) were automatically loaded into the VITEK 2 ID system (bioMérieux), using GP ID REF21342 and GN ID REF21341 cards for the identification of Gram-positive and Gram-negative bacteria, respectively and the version 07.01 release software. The cards were read by kinetic fluorescence measurement, and the results reported within 3 h [7]. Quality control for Vitek was done using Gram-positive bacteria (Enterococcus casseliflavus ATCC 700327 and Staphylococcus saprophyticus BAA-750) and Gram-negative bacteria (Enterobacter hormaechei ATCC 700323 and Stenotrophomonas maltophilia ATCC 17666). Skim milk growth medium (20%) was used to store the identified isolates and frozen at −20°C [8].