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Vertical rotors
Vertical rotors are fairly specialized--their most common use is during ultracentrifugation for isopycnic separations, specifically for the banding of DNA in cesium chloride.Once it is determined that a vertical rotor is appropriate for the end-user application, volume and speed become the deciding factors for which rotor to use.
Vertical rotors
Vertical rotors are fairly specialized—their most common use is during ultracentrifugation for isopycnic separations, specifically for the banding of DNA in cesium chloride. In this type of separation, the density range of the solution contains the same density as the particle of interest; thus the particles will orient within this portion of the gradient. Isopycnic separations are not dependent on the pathlength of the gradient but rather on run time, which must be sufficient for the particles to orient at the proper position within the gradient. Vertical rotors have very low K factors (typically in the range of 5–25), indicating that the particle must only travel a short distance to pellet (or in this case form a band); therefore run time is minimized. Once it is determined that a vertical rotor is appropriate for the end-user application, volume and speed become the deciding factors for which rotor to use.
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