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Zone electrophoresis is similar to moving boundary electrophoresis in that it uses a homogeneous buffer solution to separate proteins.This format usually employs a support medium or matrix to decrease convectioncurrent andavoiduncontrolled sample diffusion.Inmost cases,thematrixhas anadditional sieving action that affects electrophoretic separation.Samples for ZE separation are enclosed in an electrophoretic solution buffer and separated in the matrix for a predetermined amount of time.After the separation operation is completed, components of the sample with comparable qualities are divided into a separate zone.
Zone electrophoresis is similar to moving boundary electrophoresis in that it uses a homogeneous buffer
solution to separate proteins. This format usually employs a support medium or matrix to decrease
convectioncurrent andavoiduncontrolled sample diffusion. Inmost cases,thematrixhas anadditional
sieving action that affects electrophoretic separation. Samples for ZE separation are enclosed in
an electrophoretic solution buffer and separated in the matrix for a predetermined amount of time.
When an electric current (q) is applied to the sample, it moves at different speeds depending on its
mass and charge. After the separation operation is completed, components of the sample with
comparable qualities are divided into a separate zone.
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