لخّصلي

Pathogen detection As for other Pseudomonas that infect coffee, detection of Psta is currently based on morphological, physiological and biochemical tests.Even though Psgc, Psta and Pseu- domonas cichorii (Pch) can be clearly distinguished using rpoD-based phylogenetic analysis after a somewhat long and cumbersome process that requires well-trained labo- ratory personnel (Parkinson et al., 2011; Raimundi, 2017), practical methods for detection must be devel- oped.

Pathogen detection As for other Pseudomonas that infect coffee, detection of Psta is currently based on morphological, physiological and biochemical tests. Even though Psgc, Psta and Pseu- domonas cichorii (Pch) can be clearly distinguished using rpoD-based phylogenetic analysis after a somewhat long and cumbersome process that requires well-trained labo- ratory personnel (Parkinson et al., 2011; Raimundi, 2017), practical methods for detection must be devel- oped. Attempts to develop simple detection methods
based on the polymorphisms present in the rpoD locus have not been reported. Detection methods could also be based on initial comparisons of the genomes of strains that belong to the three coffee pathogens that are avail- able in the databases to identify DNA regions or bio- chemical functions specific to each one of them. The establishment of such methods could help determine the real impact of the disease in coffee plantations