Lakhasly

1.The absorbance readings of the gallic acid standards are used to create a calibration curve (Absorbance vs. Concentration).Measurement: The intensity of the blue color is measured using a spectrophotometer at a wavelength of 765 nm.

Calculation and Results:

A standard curve is prepared using known concentrations of Gallic Acid (a common phenolic standard), ranging from 0 to 1.0 mg/mL.The TPC of the LSPE sample is calculated using the slope of this standard curve and is expressed as milligrams of Gallic Acid Equivalents per 100 grams of extract (mg GAE/100 g extract).Procedure:

Mixing: 0.2 mL of LSPE is mixed with 0.4 mL of Folin-Ciocalteu (FC) reagent.

1. Total Phenolic Content (TPC) Assay
   Purpose: To quantify the total amount of phenolic compounds in the LSPE extract. Phenolics are antioxidants, so a higher TPC generally indicates higher antioxidant potential.

Procedure:

Mixing: 0.2 mL of LSPE is mixed with 0.4 mL of Folin-Ciocalteu (FC) reagent.

Reaction Time: The mixture is left at room temperature for 5 minutes.

Basification: 1 mL of sodium carbonate solution is added. This step creates a basic environment, which is necessary for the color development reaction.

Incubation: The final mixture is incubated at room temperature for 2 hours. During this time, a blue color develops.

Measurement: The intensity of the blue color is measured using a spectrophotometer at a wavelength of 765 nm.

Calculation and Results:

A standard curve is prepared using known concentrations of Gallic Acid (a common phenolic standard), ranging from 0 to 1.0 mg/mL. These standards are treated in the exact same way as the LSPE sample.

The absorbance readings of the gallic acid standards are used to create a calibration curve (Absorbance vs. Concentration).

The TPC of the LSPE sample is calculated using the slope of this standard curve and is expressed as milligrams of Gallic Acid Equivalents per 100 grams of extract (mg GAE/100 g extract). This means the phenolics in LSPE are as effective as X mg of gallic acid.