Lakhasly

Effect of Camel Milk on the Expression of Oxidative Stress Genes in HepG2 Cells To further explore whether camel milk-induced apoptotic genes is associated with induction of oxidative stress markers in HepG2 cells, we examined the effect of camel milk on heme oxygenase 1 (HO-1) and NQO1 mRNAs and ROS production as oxidative stress marker.Figure 3(a) shows that camel milk significantly induced HO-1 mRNA levels only at the higher concentrations tested, 10 and 20 mg/mL, by approximately 3 and 4 folds, respectively.

Effect of Camel Milk on the Expression of Oxidative Stress Genes in HepG2 Cells
To further explore whether camel milk-induced apoptotic genes is associated with induction of oxidative stress markers in HepG2 cells, we examined the effect of camel milk on heme oxygenase 1 (HO-1) and NQO1 mRNAs and ROS production as oxidative stress marker. For this purpose, HepG2 cells were incubated for indicated time points with increasing concentrations of camel milk, thereafter HO-1 and NQO1 mRNA and ROS production were measured by RT-PCR and DCF assay, respectively. Figure 3(a) shows that camel milk significantly induced HO-1 mRNA levels only at the higher concentrations tested, 10 and 20 mg/mL, by approximately 3 and 4 folds, respectively. In addition, while camel milk did not alter the expression of NQO1 mRNA levels (Figure 3(b)), it significantly increased the ROS production at all concentrations tested in a concentration-dependent manner (Figure 3(c)) with a maximum induction of 10-fold achieved by 20 mg/mL camel milk treatment.