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The working principle of the device:
The device separates the components of the sample, then recognizes and quantifies them, and the separation is done by distributing the sample between the two phases of the moving phase (liquid) and the stationary phase (liquid or solid), depending on the different physical properties of each component, as it moves through the stationary phase at different speeds, which leads to the separation of the components from each other in the form of layers, where the time of exit of the separate components from the column is measured to identify the material and measure its concentration, usually the fixed phase is in a column about ??


Original text

The working principle of the device:
The device separates the components of the sample, then recognizes and quantifies them, and the separation is done by distributing the sample between the two phases of the moving phase (liquid) and the stationary phase (liquid or solid), depending on the different physical properties of each component, as it moves through the stationary phase at different speeds, which leads to the separation of the components from each other in the form of layers, where the time of exit of the separate components from the column is measured to identify the material and measure its concentration, usually the fixed phase is in a column about سم cm long and wide, and the separation efficiency depends on the components of the column, in particular the diameter of the molecules of the treated material, and the that reducing the diameter of the particles leads to an improvement
This is why modern devices are called liquid chromatography high pressure devices, where the pump pushes the moving phase inside the separation column, which in turn separates the components of the sample, which passes a dimension on the (estimator), as it is
When each component of the sample passes on (the estimator) a change occurs in the electrical signals that are recorded on a moving map to give a chromatograph.


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