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Summarize result (50%)

Tissue swelling may occur.
5. Toxic and volatizes at room temperature producing harmful vapour. This vapour is toxic to the eye and respiratory tract.
6. Expensive.
 Secondary fixation: Following fixation in formalin it is sometimes useful to submit the tissue to second fixative e.g. mercuric chloride for 4 hours. It provided firmer texture to the tissues and gives brilliance to the staining.
 Fixation Artefact: Formalin pigment: Insoluble brownish-black granular retractile birefringent pigment due to reaction of formalin with hemoglobin derivatives.
• Mercury pigments: Dark-brown irregular deposit.
• Fuzzy staining: Due to improper fixation.
• Prolonged fixation: Shrinkage of the tissue causes tissue separation and empty spaces.

 Formalin Pigment • Colour: Brownish black.
• Position: Extracellular granules .
• Mechanism of formation: Formic acid reacts with hemoglobin derivatives of the blood and produces acid formaldehyde haematein.
• How to avoid: Use buffered formalin.
• How to remove: Treat with 1.8% picric acid in absolute ethyl alcohol for 15 min.


Original text

Tissue swelling may occur.
5. Toxic and volatizes at room temperature producing harmful vapour. This vapour is toxic to the eye and respiratory tract.
6. Expensive.
 Secondary fixation: Following fixation in formalin it is sometimes useful to submit the tissue to second fixative e.g. mercuric chloride for 4 hours. It provided firmer texture to the tissues and gives brilliance to the staining.
 Fixation Artefact: Formalin pigment: Insoluble brownish-black granular retractile birefringent pigment due to reaction of formalin with hemoglobin derivatives.
• Mercury pigments: Dark-brown irregular deposit.
• Fuzzy staining: Due to improper fixation.
• Prolonged fixation: Shrinkage of the tissue causes tissue separation and empty spaces.


 Formalin Pigment • Colour: Brownish black.
• Position: Extracellular granules .
• Mechanism of formation: Formic acid reacts with hemoglobin derivatives of the blood and produces acid formaldehyde haematein.
• How to avoid: Use buffered formalin.
• How to remove: Treat with 1.8% picric acid in absolute ethyl alcohol for 15 min.


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