Lakhasly

The Elico spectrophotometer model SL171 from Hyderabad, India, was utilized in conjunction with 1 cm quartz cells that were matched.
Additionally, a Sartorius digital balance with a readability of 0.0001g was employed.
Reagents
All the chemicals used were of analytical quality, and the water used was distilled. A measured quantity of ammonium molybdate, weighing 1.7081 g, was dissolved in approximately 150 mL of warm water. The resulting solution, which appeared slightly milky, was allowed to cool to room temperature. It was then moved into a 250 mL volumetric flask and made up to the desired volume using water.
A clean beaker with a capacity of 100 mL was used to transfer 0.05g of sodium sulphide. The sodium sulphide was dissolved in approximately 50 mL of water, and the resulting solution was then moved into a 100 mL volumetric flask. The beaker was washed multiple times with water, and the washings were also added to the flask.
Water was added to the flask to reach the mark and dilute the solution. Before using the solution, iodometric standardization was conducted.
A quantity of 0.1145 grams of disodium hydrogen phosphate was transferred into a beaker with a volume of 150 mL. It was then dissolved in water before being transferred into a volumetric flask with a volume of 250 mL and diluted with water up to the mark. Afterward, working solutions were made by diluting 5 mL of this solution wi water up to 50 mL.
To create a solution of sulfuric acid with a concentration of 0.25N, the highly concentrated sulfuric acid, which has a concentration of approximately 36N, was mixed with water.
A straightforward method for measuring the concentration of phosphate using spectrophotometry is suggested as the preferred approach.
A set of 10 mL volumetric flasks were prepared.
In each flask, O. mL of 0.38/M ammonium molybdate, 3 mL of 0.25N sulfuric acid, and varying amounts of disodium hydrogen phosphate (ranging from 0.3 to 12.24 pm, with specific measurements of 0.1, 2, 0.3, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mL) were added. After that, each flask received 1 mL of a sodium sulphide solution with a concentration of 2.0833×10° M. The solutions were then left at room temperature for approximately 20 minutes.
The absorbances of the solutions were measured at 715 nm using water as a reference.
Sugarcane juice sample preparation
Sugarcanes were harvested from the sugar cane fields near Mysore. A laboratory crusher was used to extract juice from these sugar canes.
The resulting juice was collected and stored in a different bottle. To remove any particles, 5 mL of the same juice was diluted with 100 mL of water and filtered.
Detergent sample preparation
5 grams of washing powder from India was put into an oven and left there for approximately 30 minutes, at roughly
The ash that was obtained at a temperature of 350 °C was placed in a 150mL beaker. It was dissolved in approximately 50mL of water and made acidic by adding a small amount of diluted sulfuric acid. The beaker was then heated on a water bath for approximately 10 minutes to remove compounds like hydrogen sulphide and nitrite. The solution was filtered and the liquid that passed through the filter was moved into a 100mL flask. It was then diluted with water up to the marking on the flask and subsequently used for the analysis of its phosphate content.
Water sample? 3
A water sample was obtained from Kukkarahalli
Lake, which is situated in the city of Mysore.
The sample was passed through a filter made of cellulose membrane. The filtered liquid contains different types of phosphorous compounds such as orthophosphate, condensed phosphate (pyro, meta, and polyphosphate), and organically bound phosphorous. These compounds can exist in both soluble and suspended forms. It is common knowledge that only orthophosphate can be directly measured using
phosphomolybdate procedures. Therefore, to analyze the entire phosphate content, the pyro, tripoly, and polyphosphate compounds were transformed into orthophosphate by adding acid and heating it for 30 minutes.