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2.2.Dehydrated MacConkey broth was bought from HiMedia (India) and prepared according to the manufacturer's instructions, then distributed into a screw- capped bottle fitted with an inverted Durham tube for the determination of coliform bacteria (total coliforms, TC, and fecal coliforms, FC) by most probable number method (Rice et al., 2012).The colony-forming unit (CFU), Fungi, and Yeast were determined based on the plate-counting method using specific media as in APHA (Young et al., 2005).Sterilization is done by autoclaving at 121?C for 15 min under a pressure of 15 lb. per square inch.
2.2. Analysis of physical, chemical, and microbiological parameters for collected hospital wastewater samples Di"erent physical and chemical parameters of collected raw hospital wastewater samples were determined according to American Public Health Association (APHA) standard methods (Rice et al., 2012; Baird and Bridgewater, 2017). These parameters include pH, biochemical oxygen demand (BOD), total chemical oxygen demand (TCOD), volatile suspended solid (VSS), total suspended solids (TSS), volatile solid (VS), and total solid (TS). Microbiological parameters were determined for the e!uent of HWWTP, as shown in Table 1. Dilutions of wastewater samples were performed and varied from 10−1 to 10−7. For pathogenic bacteria characterization, MacConkey broth without crystal violet, that is a di"erential medium less selective than MacConkey medium, so it permits growth of enterococci, staphylococci, and Mycobacterium spp., was used. Dehydrated MacConkey broth was bought from HiMedia (India) and prepared according to the manufacturer’s instructions, then distributed into a screw- capped bottle fitted with an inverted Durham tube for the determination of coliform bacteria (total coliforms, TC, and fecal coliforms, FC) by most probable number method (Rice et al., 2012). Sterilization is done by autoclaving at 121°C for 15 min under a pressure of 15 lb. per square inch. The colony-forming unit (CFU), Fungi, and Yeast were determined based on the plate-counting method using specific media as in APHA (Young et al., 2005).
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