Lakhasly

Method validation

According to the AgraQuant AFM, Plus ELISA kit, the limit of detection (LOD) of AFM, in fresh milk is 89 ng/L.Louis, MO, USA) Validation of ELISA

was performed by determining the recovery and mean variation coefficient in raw milk spiked with different concentrations of AFM, (100, 250 and 500 ng/L.) and analyzing AFM, in raw milk.To determine the efficiency of the assay, a stan- dard solution of AFM, was purchased from Sigma- Aldrich (St.All experiments were repeated 5 times.

Method validation

According to the AgraQuant AFM, Plus ELISA kit, the limit of detection (LOD) of AFM, in fresh milk is 89 ng/L. The LOD of the method satisfied the maximum tolerance limit set by the FDA in the US (500 ng/L) [38]. Samples were considered to be pos- itive for AFM, if the levels exceeded the LOD of the assay.

To determine the efficiency of the assay, a stan- dard solution of AFM, was purchased from Sigma- Aldrich (St. Louis, MO, USA) Validation of ELISA

was performed by determining the recovery and mean variation coefficient in raw milk spiked with different concentrations of AFM, (100, 250 and 500 ng/L.) and analyzing AFM, in raw milk. The recovery of AFM, in spiked milk samples was 95.6% (coefficient of varia- tion: CV=1.23). 94% (CV=1.11) , and 99% (CV=1.06) for spiked concentrations of 100, 250 and 500 ng/L AFM,, respectively. All experiments were repeated 5 times. The recovery rates satisfied the guide Standard [39] The standard calibration showed excel- lent linearity, with R value of 0 999