Lakhasly

Culture establishment and shoot formation: (a) explants of Juniperus drupacea on MS medium containing 4 uM BA after 10 days of culture; (b) shoot formation on DKW medium containing 4 uM BA after 4 weeks of culture.In Vitro Explant Rooting Root initiation of the in vitro cultured explants was inspected immediately after they were subcultured on the rooting treatment media supplemented with auxins.However, all treatments were insufficient to induce rooting.

Culture establishment and shoot formation: (a) explants of Juniperus drupacea on MS medium
containing 4 µM BA after 10 days of culture; (b) shoot formation on DKW medium containing 4 µM
BA after 4 weeks of culture. Explant discoloration (browning) and necrotic zones of the explants are
common among Juniperus species. Test tube diameter = 25 mm.
3.2. In Vitro Explant Rooting
Root initiation of the in vitro cultured explants was inspected immediately after they
were subcultured on the rooting treatment media supplemented with auxins. At this rooting
stage, all of the plant growth regulators were tested in terms of their concentrations. More
specifically, IBA was tested at concentrations of 1.0, 2.0, 4.0, 8.0, 16.0, and 32.0 µM, NAA at
1.0, 2.0, 4.0, and 8.0 µM, and finally, IAA at 0.5, 1.0, 2.0, and 4.0 µM, respectively. However,
all treatments were insufficient to induce rooting. The effectiveness of the nutrient media
alone, containing no auxin, was also inadequate to stimulate root formation.