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Bioreactors for cell proliferation and differentiation
The therapeutic promise of stem cell-based technologies for the treatment of pathologies ranging from hair loss1 to blindness2 has precipitated the need for a cell-manufacturing sector to provide therapeutic allogeneic cells.Owing to the exten- sive infrastructure requirements and rigorous standards defined by regulatory agencies, the cost will likely be too burdensome for traditional hospitals and treatment centers and will manifest as centralized facilities that specialize in providing high-quality cells with verifiable characteristics.Variable designs have been employed for adherent versus non-adherent cells and to account for differ- ences in cellular responses to microenvironmental cues.
Bioreactors for cell proliferation and differentiation
The therapeutic promise of stem cell-based technologies for the treatment of pathologies ranging from hair loss1 to blindness2 has precipitated the need for a cell-manufacturing sector to provide therapeutic allogeneic cells. Owing to the exten- sive infrastructure requirements and rigorous standards defined by regulatory agencies, the cost will likely be too burdensome for traditional hospitals and treatment centers and will manifest as centralized facilities that specialize in providing high-quality cells with verifiable characteristics. However, cell-based thera- pies often require the application of vast quantities of cells (108–1010) in order to be effective. A practical limitation arises as the amount of space required to grow these large quantities of cells using standard cell culture apparatus is prohibitive. This has spawned a demand for bioreactors capable of supporting industrial-scale, ultra-high-density cell suspension cultures with controlled microenvironments, standardization, and uniformity of culture conditions in order to generate homogenous popula- tions of stem or lineage-specific cells. A few types of bioreactors have been employed to generate large populations of pheno- typically defined cells. Variable designs have been employed for adherent versus non-adherent cells and to account for differ- ences in cellular responses to microenvironmental cues. Table 1 summarizes several bioreactor types that have been used for cell expansion.
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