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Results
16S rDNA sequence analysis and ARDRA
A single band of about 1450 bp was obtained from all strains of P. s. pv. savastanoi isolated from olive, oleander and ash when amplifying and sequencing their 16S rDNA.Sequences of the 16S rRNA gene from bacterial strains PVBa224 (from olive), ITM313 (from oleander) and PVFiF1 (from ash) were submitted to GenBank as accession numbers AM265392, AM265391 and AM265390, respectively.Sequencing of the amplicons showed that strains from olive and oleander had only a few base changes that were not related to the species of host plant.
Results
16S rDNA sequence analysis and ARDRA
A single band of about 1450 bp was obtained from all strains of P. s. pv. savastanoi isolated from olive, oleander and ash when amplifying and sequencing their 16S rDNA. Sequencing of the amplicons showed that strains from olive and oleander had only a few base changes that were not related to the species of host plant. By contrast, all the strains from ash showed a point substitution (A/G) at position 557, originating a sequence corresponding to an extra AluI restriction site in addition to those common to strains from olive and oleander. ARDRA using AluI confirmed that ash strains were very different from olive and oleander strains.
Sequences of the 16S rRNA gene from bacterial strains PVBa224 (from olive), ITM313 (from oleander) and PVFiF1 (from ash) were submitted to GenBank as accession numbers AM265392, AM265391 and AM265390, respectively. A BLAST analysis on these sequences verified that these strains belonged to the species P. savastanoi. In fact, the results obtained showed that all three sequences AM265390, AM265391 and AM265392 clustered together with other P. s. pv. savastanoi 16S rDNA sequences present in the databases.
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