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Thelinearrangeofthemethodwasinvestigatedbydetermining calibration curves in the concentration ranges of interest.Recoveries were investigated by adding a methanol calibration solution (containing 1.0nmol for all the aldehydes)to1mLofhumanbloodcontainingknownamountsof aldehydes.Aliquots (1mL) of an aqueous solution containing ethanal, propanal, butanal, pentanal, hexanal and heptanal at concentrations of 0.01-20mM were added to 8-mL vials.The precision of the method was expressed by the relative standard deviation (%, RSD).Triplicate measurements were performed
Thelinearrangeofthemethodwasinvestigatedbydetermining calibration curves in the concentration ranges of interest. Aliquots (1mL) of an aqueous solution containing ethanal, propanal, butanal, pentanal, hexanal and heptanal at concentrations of 0.01–20mM were added to 8-mL vials. The line of best fit for the relationship between peak areas (obtainedbyintegratingtheselectedm/z181chromatograms) and concentrations of the analytes was determined by linear regression. The precision of the method was expressed by the relative standard deviation (%, RSD). To obtain the RSD values, four replicate analyses of a calibration solution containing 1mM for all aldehydes were performed. A calibration solution with low concentration (10nM) was analyzed by the present method; the detection limit was calculated on the basis of signal-to-noise (S/N) ratio¼3. Recoveries were investigated by adding a methanol calibration solution (containing 1.0nmol for all the aldehydes)to1mLofhumanbloodcontainingknownamountsof aldehydes. Triplicate measurements were performed
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