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Temperature and pH Optimum temperature for the productivity and growth of the strain ACT-A2 was determined by inoculating the organism with starch casein broth and incubation was done at 20?, 24?, 28?, 32?, and 38?Also, one of the artificial saline environments, in which the presence of halotolerant actinomycetes has been investigated is decayed monuments in which high concentrations of hygroscopic salts, including carbonates, chlorides, nitrates, sulfates, etc., are present (Saiz-Jimenez and LaizNevertheless, actinomycetes form a stable, metabolically active and persistent population in various marine ecosystems, and culture-dependent studies have revealed members of the genera Dietzia, Rhodococcus, Streptomyces, Micromonospora, Salinispora, Marinophilus, Solwaraspora, Salinibacterium, Aeromicrobium, Gordonia, Microbacterium, Mycobacterium, Nocardiopsis, Pseudonocardia, Actinomadura, Saccharopolyspora, Streptosporangium, Nonomuraea, Williamsia and Verrucosispora (Maldonado et al. 2005a; Jensen et al. 2005a; Magarvey et al. 2004; Mincer et al. 2005; Riedlinger et al. 2004).Actinomycetes isolated from marine environments have been mainly assigned to a few genera including Micromonospora,Rhodococcus and Streptomyces(Maldonado et al. 2005a).


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temp
Temperature and pH Optimum temperature for the productivity and growth of the strain ACT-A2 was determined by inoculating the organism with starch casein broth and incubation was done at 20°, 24°, 28°, 32°, and 38° C for 3 days on a gyratory shaker at 200 rpm. After incubation period, growth and production of the antibiotic were determined as described above. Similarly, optimum pH was determined by adjusting the pH of starch casein broth to values like 5, 6, 7, and 8. The pH values were adjusted by 0.1N NaOH and 0.1n HCl before sterilization (Starch et al., 2003; Vandamme et al., 1984). The incubation was done at 28°C for 3 days at 200 rpm on a gyratory shaker. Growth and production of the antibiotic were determined as described above


Actinomycetes in saline terrestrial and marine environment
Halophilic actinomycetes have been isolated from sea water, saline soils, salt lakes, brines, alkaline saline habitats and other habitats. Most of the halophilic actinomycetes have been isolated from saline soils. Also, considerable numbers of salt-tolerant and drought-resistant actinomycetes have been described from arid areas such as those of the Arabian Peninsula, Iranian Plateau, Central Asia and Australia (Al-Mueini et al. 2007). Actinomycetes isolated from marine environments have been mainly assigned to a few genera including Micromonospora,Rhodococcus and Streptomyces(Maldonado et al. 2005a). Nevertheless, actinomycetes form a stable, metabolically active and persistent population in various marine ecosystems, and culture-dependent studies have revealed members of the genera Dietzia, Rhodococcus, Streptomyces, Micromonospora, Salinispora, Marinophilus, Solwaraspora, Salinibacterium, Aeromicrobium, Gordonia, Microbacterium, Mycobacterium, Nocardiopsis, Pseudonocardia, Actinomadura, Saccharopolyspora, Streptosporangium, Nonomuraea, Williamsia and Verrucosispora (Maldonado et al. 2005a; Jensen et al. 2005a; Magarvey et al. 2004; Mincer et al. 2005; Riedlinger et al. 2004). Also, one of the artificial saline environments, in which the presence of halotolerant actinomycetes has been investigated is decayed monuments in which high concentrations of hygroscopic salts, including carbonates, chlorides, nitrates, sulfates, etc., are present (Saiz-Jimenez and Laiz


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