لخّصلي

Typical biochemistry lab have three different centrifuges: 1.Microcentrifuge: (microfuge centrifuges or mini centrifuge or bench top centrifuge) o Capacity: spinning 1-2 ml centrifuge tubes (small quantities) o Speeds: up to 12000 - 13000 rpm.High-speed centrifuge: o Speed: up to about 25,000 rpm o Capacity: various sizes, depending on the rotors (the larger the rotor, the slower the maximum speed).o Usually rotors at 4oC, so called cold centrifuge

3.2.

Typical biochemistry lab have three different centrifuges:

1. 
   

   Microcentrifuge:
   (microfuge centrifuges or mini centrifuge or bench top centrifuge)
   • Capacity: spinning 1-2 ml centrifuge tubes (small quantities) • Speeds: up to 12000 - 13000 rpm. (low to medium)
   • It has small light rotor speed up and slow down rapidly.
   • used for: separation of small samples, separation of DNA, RNA and proteins that need isolation.

   
   


2. 
   

   High-speed centrifuge:
   • Speed: up to about 25,000 rpm
   • Capacity: various sizes, depending on the rotors (the larger the rotor, the slower the maximum speed).
   • Usually rotors at 4oC, so called cold centrifuge

   
   


3. 
   

   Ultracentrifuge:
   • Capacity: up to 60 ml
   • Speeds: up to 600,000 rpm (high speed).
   • can be very dangerous if maintenance is not performed regularly because of high speeds.
   • centrifuge chamber must be refrigerated and evacuated so that friction does not heat up the suspension.