Lakhasly

Abstract: Purpose: We aimed to detect the ameliorating effects of Moringa leaves extract (MLE) against Methotrexate (MTX) induced hippocampus damage.The immunohistochemical study focusing on P53 immunoreactivity showed that the contrasting findings in P53 expression between the control and MLE-treated groups suggest that MLE may modulate the expression of this tumor suppressor gene in the hippocampus.Rats were divided into to equal five groups; Group I: served as a control group, Group II: received MLE orally (300 mg/kg b w) twice a week for four weeks, Group III: injected intraperitoneal with MTX (0.5mg/kg b w) twice a week for four weeks, Group IV: rats received MTX and MLE at the same timeline for four weeks with the same dose and route of administration as mentioned previously, Group V: received MTX firstly followed by MLE for four weeks with the same dose and route of administration as mentioned previously.

Abstract:
Purpose: We aimed to detect the ameliorating effects of Moringa leaves extract (MLE) against Methotrexate (MTX) induced hippocampus damage.
Methods: This study was carried out on 50 male Albino rats weighing 150-170 g. All animals’ procedures were performed according to the principle in the guide for the care and use of laboratory animals approved by Institutional Animal Ethics Committee approval number 341/06/2022. Rats were divided into to equal five groups; Group I: served as a control group, Group II: received MLE orally (300 mg/kg b w) twice a week for four weeks, Group III: injected intraperitoneal with MTX (0.5mg/kg b w) twice a week for four weeks, Group IV: rats received MTX and MLE at the same timeline for four weeks with the same dose and route of administration as mentioned previously, Group V: received MTX firstly followed by MLE for four weeks with the same dose and route of administration as mentioned previously.

Results: the histological examination showed that in the control group, and group II showed that there was a normal hippocampal features. Conversely, Group III revealed pronounced alterations, including a contracted CA size and a C-shaped DG with a lost angle, accompanied by pyramidal cell degeneration. In the group IV, the DG angle partially restored, indicating a potential mitigating effect of MLE, yet a mixed cellular landscape persisted. In group V, showed persistent challenges in mitigating MTX induced alterations, with the contracted CA size and C-shaped DG configuration persisting. The immunohistochemical study focusing on P53 immunoreactivity showed that the contrasting findings in P53 expression between the control and MLE-treated groups suggest that MLE may modulate the expression of this tumor suppressor gene in the hippocampus.
Conclusions:in conclusion, these variations underscore the complexity of the interactions between MTX, MLE, and cellular processes within the hippocampus. Differences in dosages, timing, and the specific pathways targeted by each treatment may contribute to the observed variations in P53 immunoreactivity.